Proc. of Second World Avocado Congress 1992 pp. 559-562

 

A Study of Avocado Germplasm Resources, 1988 1990. V. The Evaluation of the Collected Avocado Germplasm Material for Horticultural Purposes

 

 

A. Ben-Ya'acov, Miriam Zilberstaine, and I. Sela

ARO, Volcani Center, Bet-Dagan, 50250, Israel

 

 

Abstract. During earlier stages of the research, germplasm was collected in different countries and planted in Israel as a germplasm bank. About 50 representatives of this germplasm bank were propagated vegetatively in 1985 and planted in selected sites under stress conditions. The soils where the experimental orchards were planted have one or more of the following stress factors: salinity, lime, limited aeration, exchangeable sodium, high pH, low fertility and Phytophthora infestation. Vegetative development and health conditions of the trees were surveyed every year. Recently, most of the trees were grafted to a commercial cultivar. The findings collected from the experimental orchards show large differences among germplasm sources when planted as rooted trees.

 

 

Growing conditions for the avocado are worsening at an accelerated rate (salinity, exchangeable Na+, Phytophthora cinnamomi infection, etc.). Thus, there is a need to continuously search for stress-tolerant rootstocks (Soderholm, 1981).

This project is a part of an international study of avocado germplasm collected from different countries, mostly areas of origin of the avocado (Ben Ya'acov et al., 1990). The germplasm collected is evaluated in Israel for horticultural purposes.

Materials and Methods

The 54 different rootstocks (Table 1) were propagated clonally and planted in 1985 in orchards at 6 sites in different commercial avocado areas of Israel. Six replicates were planted at each site. The sites are characterized by different stress conditions (Table 2). Two years after planting, the rootstocks were grafted to a commercial variety, usually Wurtz, a dwarf rootstock. Tree growth and development was investigated mainly by using aerial view photography. An annual survey of leaf burn, chlorosis and Phytophthora cinnamomi tolerance, gives us a large number of basic data on each rootstock.

Results

The information collected in the first 5 years includes one year with unusual heat (1988) and one year with severe frost (1989). During these years, four of our grafted clones are growing well in P. cinnamomi-infested soil (Table 3). The outstanding clones 'Day', 'Antigua', 'Galvan', and '256' showed no leaf burn, no degeneration symptoms and have the best growth and development rates. Eight other clones from the germplasm bank were marked as moderate and will be studied more in the future.

In the alkaline soils, 'Mayo 133' has had outstanding resistance. 'VC 257' grew well in salinity and poor aerated soils. Under the severe desert conditions in Gilat, 'Stuart' showed no leaf burn symptoms due to salinity and lime, but exhibited chlorosis. 'Day', 'Antigua1, 'Galvan1 and '256' were outstanding clones under the various stress conditions.

Discussion

It is well known that clones differ in their behavior when planted in different ecological conditions. In our study, some of the rooted types are more universal in their adaptation, such as 'Day' and 'Orizaba 3', while others grew well only in one stress environment.

The wide behavioral range of our analyzed rootstocks enables us to select specific stocks for specific stress conditions and even more important than the study of the individual physiological behavior basis for specific resistance mechanisms. Ultimately, we hope to develop a bioassay for rapid screening for a specific resistance.

Literature Cited

Ben-Ya'acov, A., G. Bufler, A. Barrientos, E. De La Cruz Torres, L. Lopez, H. Degani, and A. Solis Molina. 1990. A study of the avocado genetic resources. GIARA Project 1-15.

Soderholm, P.K. 1981. Catalog of plant germplasm available from the Subtropical Horticulture Research Unit, Miami, Florida. Agric. Rev. and Manuals, ARM-S-19.


 

Table 1. Israeli avocado germplasm bank: First list of collections planted in the orchard for evaluation.

Name

Country of origin

State or region

Type/Race

Description

Amatenango

Mexico

Chiapas

Guatamalan

Primitive type

Amatlan

Mexico

Nayarit

West-Indian

Isolated tree

Antigua

Mexico

Vera Cruz

West-Indian

Dwarfing type

Apakia 1

Ecuador

Chota Valley

Hybrid

 

Apakia 2

Ecuador

Chota Valley

Hyrbid

 

Aquilal

Mexico

Vera Cruz

Mexican

Very primitive

Aquila 2

Mexico

Vera Cruz

Mexican

Very primitive

Argui 1

Canary Isls.

G. Canary

West-Indian

 

Argui 3

Canary Isls.

G. Canary

West-Indian

 

Avocatosa 2

Mexico

Nayarit

West-Indian

Primitive, strong

Avocatosa 3

Mexico

Nayarit

West-Indian

Primitive, strong

Banios

Ecuador

Banios

Mexican

Big "Nacional"

Comitan 1

Mexico

Chiapas

Guatemalan

Primitive type

Cornitan 2

Mexico

Chiapas

Guatemalan

Primitive type

Comitan 3

Mexico

Chiapas

Guatemalan

Primitive Type

Cuevas

Mexico

Chiapas

Guatemalan

 

Day

U.S.A.

Florida

West-Indian

 

Guat.

Ecuador

Guayabamba

Not identified

 

El Charco 1

Spain

Malaga

Not identified

Old introduction

El Charco 2

Spain

Malaga

Not identified

Old introduction

El Venado

Mexico

Nayarit

Hybrid?

Very strong tree

Gainesville

U.S.A.

Florida

Mexican

Frost Resistant

Guayabamba

Ecuador

Guayabamba

Mexican

"Nacional" type

Guzman

Mexico

Nayarit

West-Indian

Drought resistant

HAES 7315

U.S.A.

Hawaii

Hybrid

Root rot resistant

KM. 145

Mexico

Chaipas

Mexican

Primitive type

La Piscina

Spain

Malaga

Not identified

Old tree

Las Posas

Spain

Malaga

Not identified

Old tree

Leyad aro

Canary Isls

Tenerife

Not identified

Very strong tree

Maskaria 1

Ecuador

Chota Valley

Not identified

 

No race

Mexico

Vera Cruz

Not identified

 

Novillero

Mexico

Vera Cruz

West-Indian

Flooding resistant

Orizava 1

Mexico

Vera Cruz

West-Indian

 

Orizaba 3

Mexico

Vera Cruz

West-Indian

 

Orizaba 4

Mexico

Vera Cruz

West-Indian

 

P. americana C2

U.S.A.

California

 

S.C.F.S., U.C.R.

P. americana H. 1 .72

U.S.A.

California

 

S.C.F.S., U.C.R.

P. americana PIS. 96 15

U.S.A.

California

 

S.C.F.S., U.C.R.

P. indica

Canary Isls.

 

 

 

P. lingue

U.S.A.

California

 

S.C.F.S., U.C.R.

P. longipes

U.S.A.

California

 

S.C.F.S., U.C.R.

P. nubigena I/7

U.S.A.

California

 

S.C.F.S., U.C.R.

P. nubigena I/8

U.S.A.

California

 

S.C.F.S., U.C.R.

P. schiedeana

Mexico

Vera Cruz

Chinini

 

San Cristobal

Mexico

Chiapas

Guatemalan

 

San Javier I

Canary Isls.

La Gomera

West-Indian

 

San Sabastian 10

Canary Isls.

La Gomera

West-Indian

 

San Sabastian 11

Canary Isls.

La Gomera

West-Indian

 

Sholola 1

Mexico

Chiapas

Guatemalan

 

Tezuitlan

Mexico

Vera Cruz

Mexican

 

Toro Blanco

Mexico

Vera Cruz

West-Indian

Flooding resistant

 


Table 2. Germplasm evaluation plots in Israel.

Location

Stress Conditions

Planted Clones (1985)

Givat-Haim

P. cinnamomi

52

Neve-Ya'ar

Salinity, lime

42

Emeq-Heffer

Salinity

30

Maoz-Haim

Exchangeable Na+

23

Bet-Dagan

Dune

28

Gilat

Salinity, lime

15

 

Table 3. The best developed trees from germplasm rooted clones in different orchards.

 

 

Orchard

V.C. No.

Name

Givat Haim

Neve Ya'ar

Maoz Haim

Emeq Hefer

Bet Degan

Gilat

202

Avocasta 2

-

.

x

x

+

-

204

Guzman

-

-

x

-

-

x

207

Day

+

+

+

+

+

+

209

Mayo 133

-

+

+

-

-

x

210

PIC 9615

-

+

-

-

-

+

213

P. americana C2

-

-

+

-

-

-

214

Dade

+

-

x

x

x

x

217

P. americana T2

-

-

+

-

x

-

218

Antigua

+ +

-

-

-

+

+

225

Galvan

+ +

-

x

+

+

-

226

Orizaba 1

-

-

+

x

x

-

227

Orizaba 4

+

+

x

-

+

x

231

Maskaria 1

-

+

-

-

-

-

232

Km 145

-

+

x

-

-

x

235

Orizaba 3

+

+

+

+

+

-

237

Sholola

-

-

-

-

+

x

239

Gainsville

+

-

x

x

x

x

240

Amatlan

-

+

x

x

x

x

241

P. nubigena 1/7

+

x

-

-

+

x

242

P. nubigena 1/8

-

x

-

-

+

x

245

P. gigantea

-

+

-

x

x

-

246

P. flocossa

-

+

x

x

x

x

247

Stuart

-

-

-

-

-

-

256

Not identified

+ +

x

x

x

x

x

Legend: (+ +) = very good, (+) = good, (-) = not good, (x) = not included in the plot.